ASCP ASCP-MLT exam : MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)

ASCP-MLT Exam Simulator
  • Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Jul 25, 2026
  • Q & A: 562 Questions and Answers

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Hematology20-25%- Hemostasis and coagulation testing
- Routine and automated hematology procedures
- White blood cell morphology and diseases
- Red blood cell physiology and disorders
Blood Banking (Immunohematology)15-20%- Transfusion reactions and adverse events
- Blood group systems and antigens
- Blood components and storage
- Compatibility testing
- Antibody detection and identification
Laboratory Operations5-10%- Laboratory safety and regulatory compliance
- Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Physical, chemical, and microscopic analysis
- Semen and amniotic fluid testing
- Cerebrospinal, serous, and synovial fluids
Immunology and Serology5-10%- Immunologic testing methodologies
- Autoimmune and immunodeficiency disorders
- Infectious disease serology
- Immune system principles and components
Microbiology15-20%- Specimen collection and culture techniques
- Bacteriology and antimicrobial susceptibility
- Mycology, parasitology, and virology
- Identification of pathogenic microorganisms
Clinical Chemistry20-25%- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
- Endocrinology and therapeutic drug monitoring
- Toxicology and liver/kidney function tests

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. The indole (modified Kovacs' reagent) is used to assess the ability of certain bacteria to produce indole by the deamination of tryptophan. Indole reacts with aldehyde in the Kovac's reagent to produce a red color. An alcoholic layer holds the red color as a ring at the top of the tube used for the idole test.
If a bacteria produces the enzyme tryptophanase to break down the amino acid tryptophan, which of the following tests will be positive with Kovac's reagent?

A) indole test
B) citrate test
C) oxidase test
D) catalase test
E) esculin test


2. Which of the following genotypes cause beta thalassemia minor?

A) B+/B
B) B0/B0
C) B/B
D) B0/B


3. Identify the following cell types by matching them with their respective image.

A) Macrophage with hemophagocytosis
B) Megakaryocyte
C) Stromal Cells
D) Osteoblast
E) Osteoclast


4. After collection all blood should be stored at 1 - 6oC, unless it is going to be used as a source of platelets.
A refrigerator used to store whole blood must be able to maintain a temperature in the ranges of:

A) 1 - 6 degrees Celsius
B) 2 - 4 degrees Celsius
C) 0 - 4 degrees Celsius
D) 2 - 8 degrees Celsius


5. This patient is most likely suffering from sickle cell anemia. This cell, which is sickle-shaped, is indicative of the presence of hemoglobin S. Polychromasia is also commonly observed in sickle cell anemia.
After experiencing crippling pain in her chest, Elizabeth's mother rushes her to the Emergency Room. After a complete blood count and differential are ordered, the hematology technologist views many peripheral cells similar in appearance to those found in the image below. Which condition is most likely present?

A) sickle cell anemia
B) hemoglobin c disease
C) myocardial infarction
D) beta thalassemia


Solutions:

Question # 1
Answer: A
Question # 2
Answer: A
Question # 3
Answer: C
Question # 4
Answer: A
Question # 5
Answer: A

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